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技術文獻:小鼠IL-1β,IL-6,等ELISA試劑盒技術文獻

發布時間: 2021/3/17  點擊次數: 192次      文件下載    

【文獻標題】Rational Avoidance of Protease Cleavage Sites and Symmetrical End-Tagging Significantly Enhances the Stability and Therapeutic Potential of Antimicrobial Peptides
【作者】Yongjie Zhu, Changxuan Shao, Guoyu Li,et.al
【作者單位】東北農業大學( Northeast Agricultural University)
【文獻中引用產品】
小鼠白介素1β (IL-1β)ELISA試劑盒
小鼠白介素6(IL-6)ELISA試劑盒
小鼠腫瘤壞死因子α(TNF-α)ELISA試劑盒
【DOI】dx.doi.org/10.1021/acs.jmedchem.0c00583
【影響因子(IF)】2.57
【出版期刊】《MED CHEM》
【產品原文引用】
In Vivo Efficacy Assay (Peritonitis-Sepsis Mode
Twenty-four C57BL/6 female mice (Liaoning Changsheng Biotechnology Co., Ltd.,Benxi, China) aged 6?7 weeks and weighing 20?22 g were randomly divided into the following three groups (n = 8): (a) control group(saline), (b) infection group (E. coli), and (c) treatment group (E. coli + II-I4-II). After 3 days of prefeeding, 100 μL of saline was injected into the peritoneal cavity of mice in the control group, while other mice received 100 μL of E. coli 25922 (~1 × 108 CFU/mL) to initiate infection. The treatment group was treated with II-I4-II (10 mg/kg) via intraperitoneal injection 1 h after infection, while the control group and infection group received saline. Then, 12 h after the infection, the mice were euthanized. At the same time, the liver, spleen, kidney, and lung of mice were taken out and weighed to analyze the bacterial load in those organs. For histopathological examination, liver, spleen, kidney, and lung sections were fixed in  4% paraformaldehyde, embedded in paraffin, and stained with hematoxylin and eosin. The level of IL-1β, IL-6, and TNF-α in the serum was analyzed with ELISA kits (Shanghai Jinma)


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